| Field Sampling of Leaf materials |
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Freeze drying of tissue |
| Grinding of leaf materials with liquid nitrogen or dry ice |
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| DNA Extraction (Various buffers, e.g. CTAB) |
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| EtBr-CsCI Gradient ultracentrifucation or electroelution |
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| Dialysis of DNA in buffer |
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| Determination of DNA concentration |
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| Storage of DNA in -80 ˚C |
